Effect of Thyroid Hormone Pre-conditioning on Intestinal and Lung Damage in The Intestinal Ischemia/Reperfusion Model
Öz
Pre-conditioning of 1-3,3 ', 5-triiodothronine (T3) or 1-tyroxine (T4)
in various experimental studies has been shown to be protective
against hepatic, brain, cardiac and renal ischemia reperfusion (I/R)
damage. Intestinal I/R injury, a critical condition usually caused by
many clinical scenarios such as shock, acute mesenteric ischemia,
sepsis, mesenteric thrombosis, or bowel transplantation. The purpose
of this study is to examine the possible protective effect of
preconditioning with T3 against damage to the small intestine and
lung tissue caused by intestinal I / R.
Male Wistar Albino rats were subjected to mesenteric ischemia (30
min) followed by reperfusion (3h). Four groups were designed, each
of contain 8 animals: sham control; I/R control; I/R +50µg/kg T3
administration; and I/R+100µg/kg T3 administration. Intestinal and
lung tissue damage was examined histopathologically.
Our results indicate that 100 µg/kg T3 preconditioning attenuates the
I/R-induced histopathological changes in intestinal and lung tissues.
Histopathological damage assessment in both lung and intestinal
tissue was found to be high in the I/R group. In the 100µg T3 treated
group, intestinal mucosa and lung injury were found to be lower than
I / R and 50µg T3 treated groups.
In conclusion, it was observed that T3-induced preconditioning
before intestinal I/R injury was histopathologically protective effect
on small intestine and lung tissue caused by intesinal ischemia.
Anahtar Kelimeler
Kaynakça
- 1. Zhu Q, He G, Wang J, Wang Y, Chen W, Guo T. Down-regulation of toll-like receptor 4 alleviates intestinal ischemia reperfusion injury and acute lung injury in mice. Oncotarget 2017;8(8):13678-13689.
- 2.Taha MO, Miranda-Ferreira R, Chang AC, Rodrigues AM, Fonseca IS, Toral LB, Cardoso MR, Simões MJ, Oliveira-Junior IS, Monteiro HP, Fagundes DJ, Taha NS, Caricati-Neto A. Effect of ischemic preconditioning on injuries caused by ischemia and reperfusion in rat intestine. Transplant Proc 2012;44(8):2304-8.
- 3. Sayan-Ozacmak H, Ozacmak VH, Turan I, Barut F, Hanci V. Pretreatment with remifentanil protects against the reduced-intestinal contractility related to the ischemia and reperfusion injury in rat. Braz J Anesthesiol 2015;65(6):483-90.
- 4. Zhang F, Tong L, Qiao H, Dong X, Qiao G, Jiang H, Sun X. Taurine attenuates multiple organ injury induced by intestinal ischemia reperfusion in rats. J Surg Res 2008;149:101-9.
- 5. He Y, Ye ZQ, Li X, Zhu GS, Liu Y, Yao WF, Luo GJ. Alpha7 nicotinic acetylcholine receptor activation attenuated intestine-derived acute lung injury. J Surg Res 2016; 201(2):258-65.
- 6. Xiong LL, Tan Y, Ma HY, Dai P, Qin YX, Yang RA, Xu YY, Deng Z, Zhao W, Xia QJ, Wang TH, Zhang YH. Administration of SB239063, a potent p38 MAPK inhibitor, alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation. Int Immunopharmacol 2016;38:54-60.
- 7. Tian XF, Yao JH, Li YH, Zhang XS, Feng BA, Yang CM, Zheng SS. Effect of nuclear factor kappa B on intercellular adhesion molecule-1 expression and neutrophil infiltration in lung injury induced by intestinal ischemia/reperfusion in rats. World J Gastroenterol 2006; 12(3):388-92.
- 8. Jaeschke H. Role of reactive oxygen species in hepatic ischemia-reperfusion injury and preconditioning. J Invest Surg 2003;16:127-40.
- 9. Madesh M, Hajnoczky G. VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome C release. J Cell Biol 2001; 155:1003- 15.
- 10. Kim SM, Kim SW, Jung YJ, Min SI, Min SK, Kim SJ, Ha J. Preconditioning with thyroid hormone (3,5,3-triiodothyronine) prevents renal ischemiareperfusion injury in mice. Surgery 2014;155(3):554-61.
- 11. Li F, Lu S, Zhu R, Zhou Z, Ma L, Cai L, Liu Z. Heme oxygenase-1 is induced by thyroid hormone and involved in thyroid hormone preconditioninginduced protection against renal warm ischemia in rat. Mol Cell Endocrinol 2011;339(1-2):54-62.
- 12. Tapia G1, Santibáñez C, Farías J, Fuenzalida G, Varela P, Videla LA, Fernández V. Kupffer-cell activity is essential for thyroid hormone rat liver preconditioning. Mol. Cell. Endocrinol 2010;323 (2):292–297.
- 13. Taki-Eldin A, Zhou L, Xie HY, Chen KJ, Yu D, He Y, Zheng SS. Triiodothyronine attenuates hepatic ischemia/reperfusion injury in a partial hepatectomy model through inhibition of proinflammatory cytokines, transcription factors, and adhesion molecules. J Surg Res 2012;178(2):646-56.
- 14. Pantos C, Malliopoulou V, Mourouzis I, Thempeyioti A, Paizis I, Dimopoulos A, Saranteas T, Xinaris C, Cokkinos DV. Hyperthyroid hearts display a phenotype of cardioprotection against ischemic stress: a possible involvement of heat shock protein 70. Horm Metab Res 2006;38 (5):308–31.
- 15. Genovese T, Impellizzeri D, Ahmad A, Cornelius C, Campolo M, Cuzzocrea S, Esposito E. Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. Brain Res 2013; 1513:92-102.
- 16. Pirat A, Zeyneloglu P, Aldemir D, Yücel M, Ozen O, Candan S, Arslan G. Pretreatment with simvastatin reduces lung injury related to intestinal ischemia-reperfusion in rats. Anesth Analg 2006;102(1):225-32.
- 17. Hierholzer C, Kalff JC, Audolfsson G, Billiar TR, Tweardy DJ, Bauer AJ. Molecular and functional contractile sequelae of rat intestinal ischemia/reperfusion injury. Transplantation 1999;68(9):1244-54.
- 18. Fantozzi ET, Breithaupt-Faloppa AC, Ricardoda-Silva FY, Rodrigues-Garbin S, Romero DC, da Silva Rodrigues A, Riffo-Vasquez Y, Tavares-deLima W. Estradiol mediates the long-lasting lung inflammation induced by intestinal ischemia and reperfusion. J Surg Res 2018;221:1-7.
- 19. Bonservizi WG, Koike MK, Saurim R, Felix GA, da Silva SM, Montero EF, Taha MO. Ischemic preconditioning and atenolol on lung injury after intestinal ischemia and reperfusion in rats. Transplant Proc 2014;46(6):1862-6.
- 20. Han X, Yao W, Liu Z, Li H, Zhang ZJ, Hei Z, Xia Z. Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1/Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxid Med Cell Longev 2016;2016:1-12.
- 21. Oliveira TRR, Oliveira GF, Simões RS, Feitosa SM, Tikazawa EH, Monteiro HP, Fagundes DJ, Taha MO. The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline. Acta Cir Bras 2017;32(11):935-948.
- 22. Wang Z, Ji Y, Wang S, Wang R, Li Z, Kang A, Xu H, Shi M, Zhao M. Protective effect of intestinal ischemic preconditioning on ischemia reperfusioncaused lung injury in rats. Inflammation 2015;38(1):424-32.
- 23. Xue TM, Tao LD, Zhang J, Xue TM, Tao LD, Zhang J, Zhang PJ, Liu X, Chen GF, ZHU, YJ. Intestinal ischemic preconditioning reduces liver ischemia reperfusion injury in rats. Mol Med Rep. 2016;13(3):2511-7.
- 24. Ji YY, Wang ZD, Wang SF, Wang BT, Yang ZA, Zhou XR, Lei NN, Yue WN. Ischemic preconditioning ameliorates intestinal injury induced by ischemia-reperfusion in rats. World J Gastroenterol 2015;21:8081-8.
- 25. Fernández V, Castillo I, Tapia G, Romanque P, Uribe-Echevarría S, Uribe M, Cartier-Ugarte D. Thyroid hormone preconditioning: Protection against ischemia-reperfusion liver injury in the rat. Hepatology 2007;45:170-177.
- 26. Vargas R, Videla LA. Thyroid hormone suppresses ischemia-reperfusion-induced liver NLRP3 inflammasome activation: Role of AMPactivated protein kinase. Immunol Lett 2017;184:92- 97
İntestinal İskemi/Reperfüzyon Modelinde Tiroid Hormon Ön Koşullanmasının İnce Bağırsak ve Akciğer Hasarı Üzerine Etkisi
Öz
Çeşitli deneysel çalışmalarda l-3,3′,5- triiodotronin (T3) veya ltiroksin
(T4) önkoşullanmasının karaciğer, beyin, kalp ve böbrek
iskemi reperfüzyon (İ/R) hasarına karşı koruyucu etkili olduğu
gösterilmiştir. İntestinal I/R hasarı, genellikle şok, akut mezenterik
iskemi, sepsis, mezenterik tromboz veya bağırsak nakli gibi birçok
klinik durumun neden olduğu kritik bir tablodur. Bu çalışmanın
amacı T3 ile oluşturulan önkoşullanmanın intestinal İ/R ile oluşan
ince bağırsak ve akciğer dokusundaki hasara karşı olası koruyucu
etkisini incelemektir.
Erkek Wistar Albino cinsi sıçanlara 30 dakika mezenterik iskemi ve
sonrasında 3 saat reperfüzyon uygulandı. Her grupta 8 hayvan olmak
üzere 4 gruba ayrıldılar: sham kontrol, İ/R kontrol, İ/R +50µg/kg T3
uygulanan ve İ/R+100µg/kg T3 uygulanan grup. İntestinal ve
akciğer dokusundaki hasar histopatolojik olarak incelenmiştir.
Sonuçlarımız ince bağırsak ve akciğer dokusunda 100 µg/kg
dozunda T3 önkoşullanmasının İ/R ile oluşan patolojik değişikleri
azalttığını göstermektedir. İ/R grubunda hem akciğer hem de
intestinal dokuda histopatolojik hasar değerlendirmesinin yüksek
seviyesinde olduğu bulunmuştur. 100 µg İ/R +T3 uygulanan grupta
intestinal mukoza ve akciğer hasarının İ/R ve İ/R +50µg T3
uygulanan gruba göre düşük olduğu saptanmıştır.
Sonuç olarak, intestinal I/R hasarından önce T3 ile oluşturulan
önkoşullanmanın bağırsak iskemisinin neden olduğu ince bağırsak
ve akciğer dokusu üzerinde histopatolojik olarak koruyucu etkili
olduğu gözlemlendi.
Anahtar Kelimeler
Kaynakça
- 1. Zhu Q, He G, Wang J, Wang Y, Chen W, Guo T. Down-regulation of toll-like receptor 4 alleviates intestinal ischemia reperfusion injury and acute lung injury in mice. Oncotarget 2017;8(8):13678-13689.
- 2.Taha MO, Miranda-Ferreira R, Chang AC, Rodrigues AM, Fonseca IS, Toral LB, Cardoso MR, Simões MJ, Oliveira-Junior IS, Monteiro HP, Fagundes DJ, Taha NS, Caricati-Neto A. Effect of ischemic preconditioning on injuries caused by ischemia and reperfusion in rat intestine. Transplant Proc 2012;44(8):2304-8.
- 3. Sayan-Ozacmak H, Ozacmak VH, Turan I, Barut F, Hanci V. Pretreatment with remifentanil protects against the reduced-intestinal contractility related to the ischemia and reperfusion injury in rat. Braz J Anesthesiol 2015;65(6):483-90.
- 4. Zhang F, Tong L, Qiao H, Dong X, Qiao G, Jiang H, Sun X. Taurine attenuates multiple organ injury induced by intestinal ischemia reperfusion in rats. J Surg Res 2008;149:101-9.
- 5. He Y, Ye ZQ, Li X, Zhu GS, Liu Y, Yao WF, Luo GJ. Alpha7 nicotinic acetylcholine receptor activation attenuated intestine-derived acute lung injury. J Surg Res 2016; 201(2):258-65.
- 6. Xiong LL, Tan Y, Ma HY, Dai P, Qin YX, Yang RA, Xu YY, Deng Z, Zhao W, Xia QJ, Wang TH, Zhang YH. Administration of SB239063, a potent p38 MAPK inhibitor, alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation. Int Immunopharmacol 2016;38:54-60.
- 7. Tian XF, Yao JH, Li YH, Zhang XS, Feng BA, Yang CM, Zheng SS. Effect of nuclear factor kappa B on intercellular adhesion molecule-1 expression and neutrophil infiltration in lung injury induced by intestinal ischemia/reperfusion in rats. World J Gastroenterol 2006; 12(3):388-92.
- 8. Jaeschke H. Role of reactive oxygen species in hepatic ischemia-reperfusion injury and preconditioning. J Invest Surg 2003;16:127-40.
- 9. Madesh M, Hajnoczky G. VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome C release. J Cell Biol 2001; 155:1003- 15.
- 10. Kim SM, Kim SW, Jung YJ, Min SI, Min SK, Kim SJ, Ha J. Preconditioning with thyroid hormone (3,5,3-triiodothyronine) prevents renal ischemiareperfusion injury in mice. Surgery 2014;155(3):554-61.
- 11. Li F, Lu S, Zhu R, Zhou Z, Ma L, Cai L, Liu Z. Heme oxygenase-1 is induced by thyroid hormone and involved in thyroid hormone preconditioninginduced protection against renal warm ischemia in rat. Mol Cell Endocrinol 2011;339(1-2):54-62.
- 12. Tapia G1, Santibáñez C, Farías J, Fuenzalida G, Varela P, Videla LA, Fernández V. Kupffer-cell activity is essential for thyroid hormone rat liver preconditioning. Mol. Cell. Endocrinol 2010;323 (2):292–297.
- 13. Taki-Eldin A, Zhou L, Xie HY, Chen KJ, Yu D, He Y, Zheng SS. Triiodothyronine attenuates hepatic ischemia/reperfusion injury in a partial hepatectomy model through inhibition of proinflammatory cytokines, transcription factors, and adhesion molecules. J Surg Res 2012;178(2):646-56.
- 14. Pantos C, Malliopoulou V, Mourouzis I, Thempeyioti A, Paizis I, Dimopoulos A, Saranteas T, Xinaris C, Cokkinos DV. Hyperthyroid hearts display a phenotype of cardioprotection against ischemic stress: a possible involvement of heat shock protein 70. Horm Metab Res 2006;38 (5):308–31.
- 15. Genovese T, Impellizzeri D, Ahmad A, Cornelius C, Campolo M, Cuzzocrea S, Esposito E. Post-ischaemic thyroid hormone treatment in a rat model of acute stroke. Brain Res 2013; 1513:92-102.
- 16. Pirat A, Zeyneloglu P, Aldemir D, Yücel M, Ozen O, Candan S, Arslan G. Pretreatment with simvastatin reduces lung injury related to intestinal ischemia-reperfusion in rats. Anesth Analg 2006;102(1):225-32.
- 17. Hierholzer C, Kalff JC, Audolfsson G, Billiar TR, Tweardy DJ, Bauer AJ. Molecular and functional contractile sequelae of rat intestinal ischemia/reperfusion injury. Transplantation 1999;68(9):1244-54.
- 18. Fantozzi ET, Breithaupt-Faloppa AC, Ricardoda-Silva FY, Rodrigues-Garbin S, Romero DC, da Silva Rodrigues A, Riffo-Vasquez Y, Tavares-deLima W. Estradiol mediates the long-lasting lung inflammation induced by intestinal ischemia and reperfusion. J Surg Res 2018;221:1-7.
- 19. Bonservizi WG, Koike MK, Saurim R, Felix GA, da Silva SM, Montero EF, Taha MO. Ischemic preconditioning and atenolol on lung injury after intestinal ischemia and reperfusion in rats. Transplant Proc 2014;46(6):1862-6.
- 20. Han X, Yao W, Liu Z, Li H, Zhang ZJ, Hei Z, Xia Z. Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1/Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxid Med Cell Longev 2016;2016:1-12.
- 21. Oliveira TRR, Oliveira GF, Simões RS, Feitosa SM, Tikazawa EH, Monteiro HP, Fagundes DJ, Taha MO. The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline. Acta Cir Bras 2017;32(11):935-948.
- 22. Wang Z, Ji Y, Wang S, Wang R, Li Z, Kang A, Xu H, Shi M, Zhao M. Protective effect of intestinal ischemic preconditioning on ischemia reperfusioncaused lung injury in rats. Inflammation 2015;38(1):424-32.
- 23. Xue TM, Tao LD, Zhang J, Xue TM, Tao LD, Zhang J, Zhang PJ, Liu X, Chen GF, ZHU, YJ. Intestinal ischemic preconditioning reduces liver ischemia reperfusion injury in rats. Mol Med Rep. 2016;13(3):2511-7.
- 24. Ji YY, Wang ZD, Wang SF, Wang BT, Yang ZA, Zhou XR, Lei NN, Yue WN. Ischemic preconditioning ameliorates intestinal injury induced by ischemia-reperfusion in rats. World J Gastroenterol 2015;21:8081-8.
- 25. Fernández V, Castillo I, Tapia G, Romanque P, Uribe-Echevarría S, Uribe M, Cartier-Ugarte D. Thyroid hormone preconditioning: Protection against ischemia-reperfusion liver injury in the rat. Hepatology 2007;45:170-177.
- 26. Vargas R, Videla LA. Thyroid hormone suppresses ischemia-reperfusion-induced liver NLRP3 inflammasome activation: Role of AMPactivated protein kinase. Immunol Lett 2017;184:92- 97
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 29 Aralık 2017 |
| Kabul Tarihi | 1 Kasım 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: 1 Sayı: 3 |
Zonguldak Bülent Ecevit Üniversitesi Tıp Fakültesi’nin bilimsel yayım organıdır.
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